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HISTORY
JOHNE'S INFORMATION CENTER - University of Wisconsin Ñ School of Veterinary Medicine

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Colonies of M. paratuberculosis
on culture medium without Tween
Colonies of M. paratuberculosis grown on Middlebrook agar media without Tween are rough in appearance. Tween 80 is a surfactant commonly used in culture media to accelerate the growth of mycobacteria. For M. paratuberculosis, Tween 80 can alter the colonial and cellular morphology.

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