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JOHNE'S INFORMATION CENTER - University of Wisconsin Ñ School of Veterinary Medicine
JOHNE'S INFORMATION CENTER - University of Wisconsin - School of Veterinary Medicine

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Monday, November 20, 2017
Viable MAP found in calf milk replacer

Calf milk replacer (CMR) is essentially powdered formula for baby calves. A research team led by Dr. Irene Grant tested 83 commercial CMR products obtained from dairy farms around the United States for MPA using by peptide-mediated magnetic separation (PMS)-phage assay, PMS followed by liquid culture (PMS-culture), and direct IS900 quantitative PCR (qPCR). Conventional microbiological analyses for total mesophilic bacterial counts, coliforms, Salmonella , coagulase-negative staphylococci, streptococci, nonhemolytic Corynebacterium spp., and Bacillus spp. were also performed to assess the overall microbiological quality of the CMR. Twenty-six (31.3%) of the 83 CMR samples showed evidence of the presence of MAP. Seventeen (20.5%) tested positive for viable MAP by the PMS-phage assay, with plaque counts ranging from 6 to 1,212 pfu/50 mL of reconstituted CMR (average 248.5 pfu/50 mL). Twelve (14.5%) CMR samples tested positive for viable MAP by PMS-culture; isolates from all 12 of these samples were subsequently confirmed by whole-genome sequencing to be different cattle strains of MAP. Seven (8.4%) CMR samples tested positive for MAP DNA by IS900 qPCR. Four CMR samples tested positive by both PMS-based tests and 5 CMR samples tested positive by IS900 qPCR plus one or other of the PMS-based tests, but only one CMR sample tested positive by all 3 MAP detection tests applied. All conventional microbiology results were within current standards for whole milk powders. A significant association existed between higher total bacterial counts and presence of viable MAP indicated by either of the PMS-based assays. This represents the first published report of the isolation of viable MAP from CMR. These findings further raise concerns about the potential ability of MAP to survive manufacture of dried milk-based products.

This Open Access article is available on the Journal of Dairy Science website.


Wednesday, October 25, 2017
14th International Colloquium on Paratuberculosis

In 2018, Mexico will host the 14th Colloquium of the International Association for Paratuberculosis (14-ICP) in the Riviera Maya, Quintana Roo. This 14-ICP will assemble once again a group of researchers from throughout the world. This will be the 35th anniversary of the first meeting on paratuberculosis held in Ames, Iowa, and the 28th anniversary of the founding of the International Association for Paratuberculosis. Cancun-Riviera Maya is one of the most dynamic cultural capitals, rich in history and alive with excitement and friendliness people, where visitors each year come from all parts of the world, making this a wonderful showcase for all that Mexico has to offer.

The November 15, 2017 deadline to submit abstracts for the 14th ICP is fast approaching! Please visit the meeting website to register for the conference and submit your abstracts.

Meeting website


Wednesday, September 27, 2017
The Consensus from the Mycobacterium avium ssp. paratuberculosis (MAP) Conference 2017

On March 24 and 25, 2017 researchers and clinicians from around the world met at Temple University in Philadelphia to discuss the current knowledge of Mycobacterium avium ssp. paratuberculosis (MAP) and its relationship to human disease. The conference was held because of shared concern that MAP is a zoonotic bacterium that poses a threat not only to animal health but also human health. The conference proceedings may be viewed at www.Humanpara.org.

A summary of the salient work in this field is followed by recommendations from a majority of the conferees. A majority of the conferees strongly urge that the possibility that MAP causes human disease no longer be ignored. Should further compelling evidence become available, it is recommended that the FDA and USDA (and their counterparts in other nations) have contingency plans in place to rapidly eliminate MAP from the milk and meat supply through effective MAP control measures including biosecurity and hygiene, vaccination, and test-and-cull programs. Even if public health measures are not put in place by the appropriate regulatory agencies, food producers are encouraged to offer food products from animals in MAP control programs. Many food producers are already undertaking voluntary control practices and this effort is encouraged and commended. The international representation in the authorship of this article attests to the observation that Crohn’s Disease is now a worldwide epidemic.

The article has 70 coauthors, including the past (Collins) and current (Juste) Presidents of the International Association for Paratuberculosis. This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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