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JOHNE'S INFORMATION CENTER - University of Wisconsin Ñ School of Veterinary Medicine
JOHNE'S INFORMATION CENTER - University of Wisconsin - School of Veterinary Medicine

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Thursday, January 11, 2018

Pathogenesis, Molecular Genetics, and Genomics of Mycobacterium avium subsp. paratuberculosis, the Etiologic Agent of Johne’s Disease

This excellent review article is a technical summary of what we know about MAP, mainly from the veterinary perspective. It highlights the fecal-oral method of primary transmission, and how MAP causes disease in animals by increasing intestinal permeability and infecting macrophages, which results in granulomas. Diagnostics and current vaccines are discussed, with the caveat that none provide long term immunity. Zoonotic potential is outlined, with a quick overview of published research, and the mapping of the MAP K-10 genome is presented. The article concludes with an in depth discussion of potential vaccines in development and states that live-attenuated vaccines are the best approach against mycobacteria.

This article is available in full on the Frontiers in Veterinary Science journal.


Monday, November 20, 2017
Viable MAP found in calf milk replacer

Calf milk replacer (CMR) is essentially powdered formula for baby calves. A research team led by Dr. Irene Grant tested 83 commercial CMR products obtained from dairy farms around the United States for MPA using by peptide-mediated magnetic separation (PMS)-phage assay, PMS followed by liquid culture (PMS-culture), and direct IS900 quantitative PCR (qPCR). Conventional microbiological analyses for total mesophilic bacterial counts, coliforms, Salmonella , coagulase-negative staphylococci, streptococci, nonhemolytic Corynebacterium spp., and Bacillus spp. were also performed to assess the overall microbiological quality of the CMR. Twenty-six (31.3%) of the 83 CMR samples showed evidence of the presence of MAP. Seventeen (20.5%) tested positive for viable MAP by the PMS-phage assay, with plaque counts ranging from 6 to 1,212 pfu/50 mL of reconstituted CMR (average 248.5 pfu/50 mL). Twelve (14.5%) CMR samples tested positive for viable MAP by PMS-culture; isolates from all 12 of these samples were subsequently confirmed by whole-genome sequencing to be different cattle strains of MAP. Seven (8.4%) CMR samples tested positive for MAP DNA by IS900 qPCR. Four CMR samples tested positive by both PMS-based tests and 5 CMR samples tested positive by IS900 qPCR plus one or other of the PMS-based tests, but only one CMR sample tested positive by all 3 MAP detection tests applied. All conventional microbiology results were within current standards for whole milk powders. A significant association existed between higher total bacterial counts and presence of viable MAP indicated by either of the PMS-based assays. This represents the first published report of the isolation of viable MAP from CMR. These findings further raise concerns about the potential ability of MAP to survive manufacture of dried milk-based products.

This Open Access article is available on the Journal of Dairy Science website.


Wednesday, October 25, 2017
14th International Colloquium on Paratuberculosis

In 2018, Mexico will host the 14th Colloquium of the International Association for Paratuberculosis (14-ICP) in the Riviera Maya, Quintana Roo. This 14-ICP will assemble once again a group of researchers from throughout the world. This will be the 35th anniversary of the first meeting on paratuberculosis held in Ames, Iowa, and the 28th anniversary of the founding of the International Association for Paratuberculosis. Cancun-Riviera Maya is one of the most dynamic cultural capitals, rich in history and alive with excitement and friendliness people, where visitors each year come from all parts of the world, making this a wonderful showcase for all that Mexico has to offer.

The November 15, 2017 deadline to submit abstracts for the 14th ICP is fast approaching! Please visit the meeting website to register for the conference and submit your abstracts.

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