University of Wisconsin–Madison

Mycobactin Dependency

MAP requires a substance called mycobactin to grow in the laboratory setting. The organism therefore can be identified by using two types of media: one with mycobactin, one without it. If the bacteria grows on both types of media, the organism is not mycobactin dependent and thus is not MAP. If it grows only on the media with mycobactin, this is strong evidence that the organism is MAP.