NEWS

Researchers in Northern Ireland have analyzed the responses to a Veterinary Risk Assessment and Management Plan (VRAMP) concerning Johne’s disease for 1,569 dairy herds. Their findings are reported in the recent issue of Veterinary Record and the article is Open Access.

ABSTRACT
(British spellings)

Background
Animal Health and Welfare Northern Ireland has been enrolling dairy herds across Northern Ireland (NI) in a voluntary Johne's disease (JD) control programme since October 2020. A Veterinary Risk Assessment and Management Plan (VRAMP) questionnaire was completed for each herd enrolled and recommendations for improved farm management practices were provided to farmers. Herd JD testing was recommended but was not mandatory.

Methods
This study analysed VRAMP responses for 1569 dairy herds that had enrolled in the JD control programme up to October 2022. Univariate and multivariate regression models were applied to the data as appropriate.

Results
Overall, 21.4% of the dairy herds had completed herd JD screening, with 13.7% of herds reporting a confirmed case of JD. A further 31.5% of herds reported suspected case(s) of JD. Eighty-nine percent of farms had introduced animals from outside the herd. Herds that utilise a mixed calving pen and hospital pen, and herds that do not separate JD-positive or sick animals within the calving pen, were significantly (p > 0.001) more likely to be a high-probability JD herd. Accidental mixing of neighbouring herds significantly (p = 0.01) increased the risk of a suspected or confirmed case of JD. Herds that utilise rented land (70%) were significantly (p > 0.001) more likely to be at a high risk for JD.

Conclusions
The VRAMP analysis identified areas of JD control that should be focused on in NI dairy herds, such as calving pen management and hygiene. The results highlight the importance of common JD recommendations in the management of on-farm disease risk.

COMMENT

This report yet again illustrates that purchase of animals from other herds is a common and risky practice for the introduction of infectious diseases like Johne’s disease. The second common and risky practice on dairy farms is housing sick cows and JD test-positive cows in pens where calves are being born which further facilitates infection spread to the most susceptible animals on the farm and animals likely to join the herd as replacements in the future. These risky practices have been associated with higher MAP-infection rates in dairy herds in multiple studies in other countries.

Many countries have standardized risk assessment tools for Johne’s disease in dairy cattle herds and for beef cattle herds in some countries. In the U.S. the risk assessments for both dairy and beef herd can be done using an App for iPads making completion of the task quicker and providing the option to share the result with regulatory veterinary agencies. While similar risk assessments may not be available for goats and sheep, the same basic principles apply, and veterinarians can use these concepts to help animal owners develop the most effective JD control programs for their specific situation. Contact your local veterinarian.

Researchers reported on an 11-year study of MAP transmission in 6 commercial dairy herds in the UK. Their Open Access publication appears in the October 2023 issue of Preventive Veterinary Medicine. The study is novel for its length and detailed analysis of MAP transmission. The findings largely support what has previously been reported and helps focus producers and veterinarians on the best control measures.

ABSTRACT

Johne's disease (JD) is a chronic disease of ruminants endemic in the UK and other countries and responsible for large economic losses for the dairy sector. JD is caused by Mycobacterium avium subspecies paratuberculosis (MAP), which typically infects calves that remain latently infected during a long period, making early detection of infection challenging. Cow to calf transmission can occur in-utero, via milk/colostrum or faecal-orally. Understanding of the different transmission routes to calves is important in informing control recommendations. Our aim in this longitudinal study was to measure the association between the transmission routes via the dam and the environment on a calf subsequently testing serologically positive for MAP. The study population comprised of 439 UK dairy calves from 6 herds enrolled between 2012 and 2013. These calves were followed up from birth until 2023. At birth individual calf data was captured. During follow-up, individuals entering the milking herd were quarterly tested for the presence of MAP antibodies using milk ELISA. Cox regression models were used to measure the association between exposure from the dam (in-utero and/or colostrum) or from the environment (long time in dirty yard) and time to first detection of MAP infection. An association between calves born to positive dams and probability of having a MAP positive test result remained after excluding potential MAP transmission via colostrum (Hazard ratio: 2.24; 95% CI: 1.14 – 4.41). Calves unlikely to be infected with MAP via the in-utero or colostrum route, had 3.68 (95% CI: 3.68 1.45–9.33) higher hazard of a positive test result when they stayed longer in a dirty calving area. The effect of the dam infection status on transmission to calves precedes the dam's seroconversion and persists after excluding the potential role of transmission via colostrum . The association between time spent in a dirty calving area and probability of a MAP positive test result highlights the role of environmental contamination as a source of infection in addition to the dam.

COMMENTS

This study is a noteworthy contribution to understanding the epidemiology of MAP infections in commercial dairy herds. The findings are consistent with those from other countries and the article provides a concise list of pertinent references. While this scientific publication is rather technical in nature, due to the nature of the statistical analysis, the “take home messages” are clear:

• MAP infection risk is partly driven by the dam’s infection status.
• Calves from infected dams have higher MAP infection risk, regardless of dam's test status at calving.
• Spending prolonged time in a dirty yard increases the risk of MAP infection.
• Dam's impact on MAP risk extends beyond colostrum transmission.
• MAP persistence in commercial dairy herds results from a combination of dam-related and environment-related factors.

July 18, 2023, Italian researchers published a study on paratuberculosis in 33 dairy goat farms in northern Italy. Their study explored associations between animal welfare and herd biosecurity with the rate of MAP infections in each herd as detected by a commercial serum ELISA kit. The study was published in the journal Animals and is Open Access.

ABSTRACT

Paratuberculosis is a notable infectious disease of ruminants. Goats appear to be particularly susceptible. The survey aimed to investigate the spread of paratuberculosis in Italian goat farming and evaluate whether the presence of the disease could be influenced by welfare and biosecurity deficiencies. A serological survey for paratuberculosis in 33 dairy farms in northern Italy was conducted. Contextually, animal welfare and biosecurity were assessed, using a standardized protocol of 36 welfare indicators and 15 biosecurity indicators which assigns to each farm a welfare and biosecurity score from 0 (any application) to 100% (full application). An overall result of less than 60% was considered insufficient. Nineteen farms (58%) tested positive for paratuberculosis, with a mean intra-herd seroprevalence of 7.4%. Total welfare ranged from 39.56 to 90.7% (mean 68.64%). Biosecurity scores ranged from 10.04 to 90.01% (mean 57.57%). Eight farms (24%) showed poor welfare conditions (welfare score < 60%) and 19 (58%) an unsatisfactory biosecurity condition (biosecurity score < 60%). With respect to the explorative character of the study, an indicative association between seven welfare and biosecurity indicators and paratuberculosis seropositivity was identified. The presence of paratuberculosis in northern Italy dairy goat farms was confirmed. The welfare and biosecurity assessment protocol proved to be an accurate tool, capable of identifying critical points for managing health, welfare and productivity.

COMMENTS

Paratuberculosis is common among goat herds and yet it is an often-overlooked infectious disease problem that compromises animal health, animal productivity, and animal welfare. The finding in Italy that 58% of the 33 herds tested were positive by ELISA is consistent with similar studies in other parts of the world. Importantly, they showed a correlation between the MAP-infection status of herds and their levels of animal welfare and herd biosecurity.

The Italian findings are consistent with other such surveys of goat herds in other countries done using commercial ELISA kits. In France, researchers tested 11,847 goats over 6 months old in 105 herds finding that 55.2% of herds were seropositive with 5.9% of goats being ELISA-positive within the MAP-infected herds (Mercier, 2010). In the U.S., Pithua reported in 2012 that 9 of 25 (36%) Boer goat herds in the state of Missouri were positive for paratuberculosis using a commercial ELISA kit. A 2011 study done in Cyprus tested 72 herds that had goats only or goats kept together and sheep with 4,582 total goats tested using a commercial ELISA kit. They found that 36 of 72 (50%) of herds with goats were ELISA-positive (1 or more positive goats). Within these ELISA-positive herds, 10.3% of goats tested ELISA-positive. A study of 116 herds or Korean Black Goats found regional differences in S. Korea for the rate of ELISA-positive goat herds ranging from 18.2% to 38.2% (Lee et al., 2006). A study of goat herds in the province of Ontario Canada published in 2016 found that 16 of 29 (55.2%) randomly selected dairy goat herds were ELISA-positive.

These high goat herd infection rates are all based on commercial ELISA kits and so can legitimately be compared. Because the ELISA is less sensitive than other diagnostic tests like fecal PCR, the reported values, called apparent prevalence rates, are underestimates. The true infection rates (called true herd-level prevalence) are even higher. In the Canadian study, the ELISA detected only 15/29 (52%) of all goats that were shedding MAP in their feces.

Clearly, paratuberculosis is a problem that needs to be addressed among goat herds globally for the sake of the animals, the various types of goat industries, and consumers of goat meat and dairy products.

To limit the risk of bringing MAP infections into goat herds, owners are advised to have a good biosecurity plan.

Specifically:

• Assume any goat herd you buy from is MAP-infected until proven otherwise. You will be right over 50% of the time. Some owners are simply ignorant about Johne’s disease. Others willfully ignore the problem by intentionally never testing.
• Only buy or lease goats from herds that are 100% test-negative for paratuberculosis (all adult goats in the herd) within the past 12 months. ELISA testing is acceptable but testing by fecal PCR is far more accurate, as the Canadian study showed.
• Quarantine all purchased animals until a repeat test is done by fecal PCR and found to be negative.

PREVENTION PAYS!

A team of 11 Portuguese scientists and one MAP expert from the UK have yet again demonstrated live MAP in the blood of Crohn’s Disease (CD) patients and confirmed the association of MAP and CD. In addition, they showed that MAP was far more common in CD patients than was Escherichia coli bacteria with adherence/invasive ability (AIEC). This type of E. coli has been suggested by some scientists to be a causal agent for CD. This work was published June 7 in the journal Microoganisms.

ABSTRACT

Pathobionts, particularly Mycobacterium avium subsp. paratuberculosis (MAP) and Escherichia coli isolates with adherence/invasive ability (AIEC) have been associated with inflammatory bowel disease (IBD), particularly Crohn’s disease (CD). This study aimed to evaluate the frequency of viable MAP and AIEC in a cohort of IBD patients. As such, MAP and E. coli cultures were established from faecal and blood samples (with a total n = 62 for each) of patients with CD (n = 18), ulcerative colitis (UC, n = 15), or liver cirrhosis (n = 7), as well as from healthy controls (HC, n = 22). Presumptive positive cultures were tested by polymerase chain reaction (PCR), for a positive confirmation of MAP or E. coli identity. E. coli-confirmed isolates were then tested for AIEC identity using adherence and invasion assays in the epithelial cell line of Caco-2 and survival and replication assays in the macrophage cell line of J774. MAP sub-culture and genome sequencing were also performed. MAP was more frequently cultured from the blood and faecal samples of patients with CD and cirrhosis. E. coli presumptive colonies were isolated from the faecal samples of most individuals, in contrast to what was registered for the blood samples. Additionally, from the confirmed E. coli isolates, only three had an AIEC-like phenotype (i.e., one CD patient and two UC patients). This study confirmed the association between MAP and CD; however, it did not find a strong association between the presence of AIEC and CD. It may be hypothesized that the presence of viable MAP in the bloodstream of CD patients contributes to disease reactivation.

COMMENTS

This study adds to the body of literature on the association of MAP and CD and strengthens the argument that MAP is a zoonotic pathogen, i.e., one transmissible from animals to humans. For more about MAP as a zoonotic pathogen and its link to Crohn’s Disease, see this page on our website where a long list of references on the subject is provided, with direct links to the original publications.

E. Stefanova and nine colleagues in Spain have published an article on Johne’s disease pathology in goats. They contrast the pathology found in non-vaccinated goats and goats that have been vaccinated with a commercial heat-inactivated vaccine for Johne’s disease. They also describe lesions caused by bronchopneumonia and caseous lymphadenitis (CLA) plus pathology caused by assorted other disease problems. The pictures of gross pathology are particularly good. Mesenteric lymph nodes were a the most valuable tissue to sample for detection of MAP infections in goats by histopathology. This Open Access article was published in the journal Animals on May 19, 2023.

SUMMARY

Paratuberculosis (PTB), also called Johne’s disease, is a well-known disease with considerable financial impact on the farm industry worldwide. Nevertheless, data regarding the assessment of naturally infected goat herds is limited. The present study describes in detail the observed gross and histological lesions detected in 39 necropsies of goats (15 vaccinated and 24 non-vaccinated) from herds with a confirmed history of PTB. PTB microscopic lesions of various grades were detected in all animals in target organs and the presence of the causative agent was confirmed using different laboratory tools. The main inflammatory findings affected the hemolymphatic, respiratory and gastrointestinal systems. The lesions were confirmed microscopically with lesser macroscopically visible alterations. Our result demonstrated that non-vaccinated animals presented more severe PTB intestinal lesions and had respiratory inflammation in all age groups studied. Those also presented a higher prevalence of ileocecal valve PTB lesions. Gastrointestinal non-PTB lesions were detected in higher number in non-vaccinated goats. Thus, histology is a powerful tool for herd diagnosis and assessment. Mainly inflammatory lesions of the respiratory and gastrointestinal tract were detected in the studied PTB-affected herds. Additionally, vaccination against PTB could play a key role in the reduction of lung and gastrointestinal inflammatory processes present in the herd.

COMMENTS

The Gudair® vaccine used in this study is a commercial heat-inactivated vaccine containing 2.5 mg/mL of MAP strain 316 F with mineral oil adjuvant (CZ Vaccines S.A., Spain). In Spain, it is primarily used in sheep and goats. This vaccine is not licensed for use in the United States.

R. Scarpelli together with seven Italian colleagues reported on the success of the Voluntary National Control Plan and Guidelines developed by the Italian Ministry of Health in 2013 at controlling paratuberculosis in Italian dairy herds of varying sizes.

Their publication appears in the June 2023 issue of Preventive Veterinary Medicine.

This graphic from their publication shows a steady rise in the number of ELISA negative herds (green bars), and a concomitant decrease in the number of herds with a low percentage of ELISA-positive animals (yellow bars) and number of herds with a higher percentage of ELISA-positive cows (red bars) over the time period of the study, 2017 to 2020. Interestingly, one farm that decided not to follow the proposed control program, but accepted to be sampled every year, showed an increasing rate of ELISA-positive cows (from 5.2% in 2017 to 7.6% in 2020).

ABSTRACT

Paratuberculosis is considered one of the most economically devastating infectious diseases of domestic livestock, and the most effective control strategy is a combination of ‘test-and-cull’ and on-farm biosecurity measures. In Italy, a Voluntary National Control Plan (VNCP) and guidelines have been introduced to reduce the impact of the disease, and farmers can voluntarily enroll in the control plan. The main aims of this study were: i) the description of the trend over a 4-year period on total, within-herd (WH) and between herd (BH) apparent seroprevalences observed in 64 dairy herds members of a mutual company located in Italy after the introduction of a proposed “Customized Control Plan” (CCP); ii) the evaluation of its effectiveness in terms of percentage of participating farms that decided to join the VNCP. Analyses on serum samples were performed with Enzyme-Linked ImmunoSorbent Assay (ELISA) method and revealed a general decrease in both total, WH and BH apparent seroprevalence. Total average apparent seroprevalence decreased from 2.39% in 2017 to 1% in 2020. Negative herds rose from 51.9% in 2017 to 71.1% in 2020, while farms with WH apparent seroprevalence >5% decreased from 17.3% in 2017 to 4.4% in 2020. BH apparent seroprevalence decreased from 51.2% in 2017 to 29.2% in 2020. Among the 52 out of 64 herds that accepted to continue the proposed CCP after the first year, 41 (78.8%) joined in 2020 the VNCP, that assessed the health ranking of the herds. The results provide evidence that a control plan based on a farm-specific strategy and a subsidized testing process can effectively reduce the impact of paratuberculosis in dairy herds, especially in convincing farmers to continue in paratuberculosis control by joining the VNCP, including them in a national context and increasing their awareness of the disease.

COMMENT

This study adds to the body of literature showing that paratuberculosis (Johne’s disease) in dairy cattle herds can be controlled using a cost-effective diagnostic test (ELISA) combined with herd management changes.

The 7th and 8th IDF Paratuberculosis Fora took place on 26 May 2021 and 12 June 2022 respectively. The 7th event was virtual while the 8th Forum was held in conjunction with the International Colloquium on Paratuberculosis in Dublin, Ireland, and both were chaired by Dr. David Kelton (Chair of IDF Standing Committee on Animal Health and Welfare). The participants shared insights about the current situation and control measures of their respective countries on Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis).

The Fora offered a unique opportunity for sharing past, present, and future perspectives on paratuberculosis (M. paratuberculosis, ParaTB) control, and served as a place for engaging in meaningful international discussion about how to address this globally important issue. The Fora highlighted the need for more investment in animal health, and opportunities for some harmonization and more research on factors affecting the disease progression. This publication contains a selection of eight papers, all of which were presented by program representatives on the 7th or the 8th ParaTB Fora days.

The Proceedings are available for download FREE at the IDF website.

N.L. Field and colleagues in Ireland have published their research correlating MAP detection rates in areas where dairy calves are housed and the risk assessment scores (RAMP) for the farm. The publication appears in the journal Animals 2023, 13, 669. https://doi.org/10.3390/ani13040669.

This is their summary (British spellings):
Transmission of Mycobacterium avium subspecies paratuberculosis (MAP) from infected adult cattle to susceptible calves occurs mainly through contamination of the calf environment, i.e., the calving pen and the pre-weaned calf rearing area, with adult faeces. Control programmes for Johne’s disease utilise a risk assessment and management plan (RAMP) to evaluate the risk of transmission of infection on a specific farm, however the assessment is limited by its subjective, point-in-time nature. The objective of this study was to evaluate a novel environmental sampling protocol for measurement of MAP contamination of the calf environment on infected farms. Across 28 infected farms, 46% had detectable MAP in the calf environment, with 21% of farms having detectable MAP in the pre-weaned calf area. There was no significant association found between the RAMP scores for each farm and the result of environmental testing for MAP, and there was a moderate correlation found between RAMP scores and the level of MAP contamination as quantified by PCR. We conclude that environmental sampling is a potentially useful tool to objectively measure transmission risk in the calf environment on farms, as a complement to the annual RAMP.

COMMENT

PCR has replaced culture as the most accurate, cost-effective, and quantitative method for MAP detection. It can be applied to a wide range of sample types including feces, fresh and formalinized tissues, and environmental samples. Commercially available kits provides for high reproducibility of results among laboratories. In the U.S. the laboratory proficiency assessment by USDA offers additional confidence in PCR tests for MAP. The results of the proficiency test create a list of USDA-approved laboratories available on this website. Not all labs will accept environmental samples. So, contact your veterinary diagnostic lab of choice to be sure before sending such samples.

One-hundred eighty-three years ago, on December 10, 1839, Heinrich Albert Johne was born in Dresden, Germany. It seems fitting that Johnes.org celebrate, on this date, his lasting contribution to veterinary medicine.

Below you will find the story of his discovery, a brief biography, a little about the pathogen name, and the story of how I found this photo.

How it all started.

Dr. F. Harmes, a veterinarian in the Oldenburg region of Germany in 1895, had a client with a Guernsey cow that was doing poorly.  Dr. Harmes’ preliminary diagnosis was intestinal tuberculosis (TB).  TB in cattle was quite common in Germany then.  But when he did the tuberculin skin test to confirm his diagnosis, the cow tested negative.  So, the reason for the cow’s condition remained a mystery.

A few months later, the cow died.  Curious as to what killed the cow, Dr. Harmes sent intestines and other tissues to the Pathology Unit at the veterinary school in Dresden.  There the tissues were examined by Dr. Heinrich A. Johne, Professor of Pathology, and Dr. Langdon Frothingham, a visiting scientist from the Pathology Unit in Boston, Massachusetts.

They observed that the small intestine was quite a bit thicker than expected and that lymph nodes near this thick intestine were enlarged.  The photo at the right shows a normal intestine at the top and the intestine thickened due to Johne’s disease at the bottom.  Lymphoid tissue, called Peyer’s Patches, are also quite prominent (the raised and slightly red tissue running long-ways down the center of the thickened intestine).   Interestingly, Dalziel in 1913 saw the same kind of pathology when he removed a section of intestine from a person with Crohn’s disease remarking in his report that it resembled the cattle problem Dr. Johne had described.

Using what at the time were newly developed histopathology techniques, parts of the intestine were “fixed” (pickled in formaldehyde), sliced into very thin sections, placed on a microscope slide, and stained with special dyes – known as an acid-fast stain - designed to help visualize bacteria of the type causing TB.

Under the microscope, Drs. Johne and Frothingham saw that the intestinal wall was filled with inflammatory cells of the kind to be expected in TB (macrophages and lymphocytes – the blue-colored stuff in the photo).  In addition, they saw abundant red-staining bacteria (which microbiologists call acid-fast bacteria) throughout the inflamed tissues.  Basically, it looked just like intestinal TB.  But, when a sample of the fresh infected tissue containing the red-staining bacteria was injected into guinea pigs, it didn’t cause TB.  This took place shortly after Louis Pasteur had devised the “germ theory” of disease and before techniques for growing bacteria in the laboratory were widely available.  Inoculating animals, therefore, was a routine way of detecting infectious microbes such as those that cause TB, and guinea pigs are quite susceptible to tuberculosis.  So, the diagnosis on this cow remained a mystery.

Drs. Johne and Frothingham concluded that the disease seen in the very sick Guernsey cow was caused by a bacterium other than the one normally causing TB in cattle, namely Mycobacterium bovis.  They speculated that perhaps the pathology was due to a related bacterial pathogen such as the one causing TB in birds, aptly named Mycobacterium avium.  Considering their subject’s gross pathology, microscopic pathology (histopathology) and animal inoculation findings, they proposed the name "pseudotuberculous enteritis" for the disease; a designation meaning inflammation of the intestine resembling intestinal TB but not actually the same as intestinal TB – somehow different.  Soon after publication of their report, veterinarians began reporting outbreaks of this curious intestinal malady among dairy cows in Denmark, The Netherlands and elsewhere in continental Europe.

More on Dr. Johne.

H.A. Johne was the son of a veterinarian. Twenty years later, he became a veterinarian himself and held a practice for the next seven years. From 1866-1876, he acted as district veterinary inspector. He was then appointed to a lectureship at the veterinary school in Dresden. For a teacher of veterinary medicine, he lectured in an unusually wide range of subjects: embryology, histology, obstetrics, exterior, physical diagnostics. In 1879, he was appointed professor of pathological anatomy and of general pathology. Later he also lectured on parasitology and methodical zoology, and he also started classes in such a new branch of research as bacteriology.

As a scientist, he concerned himself with tuberculosis, anthrax, rabies, glanders, actinomycosis, bothryomycosis among others. As a writer he left a wide literary production. His books were printed in a dozen editions. For many years he also edited “Zeitschrift tor Tiermedizin”, and acted as co-editor of “Rundschau auf dem Gebiet der Fleischbeschau”. In 1887, he visited Denmark, where he was nominated honorary member of the Danish Association of Veterinarians and decorated with the Order of Knight of the Dannebrog.

He was often guest of Professor B. Bang and his family, the flat of Bangs' is today the Veterinary History Museum, established in 1973 at the Royal Veterinary and Agricultural University in Copenhagen. His motto was: Duty Above All. With the distinguished array of titles: Geheim-Medizinalrat, Professor, Dr. med., Dr. med.vet.h.c. and Dr. phil., Heinrich Albert Johne retired in 1904, respected and honored by his many students and by foreign veterinary schools and societies. He died in 1910.

MAP

In 1912, in one of those curious discoveries by serendipity, Twort and Ingram discovered how to grow the cause of Johne’s disease in the laboratory and named this bacterial pathogen Mycobacterium enteritidis chronicae pseudotuberculosae bovis johne.  Time and technology led to name changes and the cause of Johne’s disease is today known as Mycobacterium avium subspecies paratuberculosis or simply MAP. Johne’s disease, also called paratuberculosis, is now a disease of major global importance.

Dr. Johne photo credit

I found the photo of Dr. Johne was hanging in halls of the State Veterinary Serum Institute when I was on sabbatical working with Dr. J.B. Jørgensen at the State Veterinary Serum Laboratory, Copenhagen, Denmark. Together we were comparing new methods for culturing MAP from clinical samples. On my departure, Dr. Jørgensen gifted me a copy of this photo which hangs in my office and also appears on the Wikipedia page about Dr. Johne.

PS

For more historical events and people important to understanding of Johne’s disease visit our history timeline.

MAP has long been associated with Crohn’s disease and many patients have benefited from anti-MAP antibiotic therapy. You can read one such story where a veterinarian was fully cured of Crohn’s disease by anti-MAP antibiotic therapy here.

Dr. Marcel Behr, Professor of Medicine at McGill University and Associate Director of the Infectious Diseases and Immunity in Global Health Program at the Research Institute of the McGill University Health Centre conducted an interview on research connecting Crohn’s disease to MAP. To me, it is one of the most thoughtful, objective assessments of the state of research on the MAP=CD hypothesis I have ever witnessed. The interview covers every aspect of the research on this association in a lucid, straight forward discussion of this controversial topic.

The interview is on You Tube. I appeared online August 13, 2022. It is 57 minutes long and, due to its technical nature, is probably best appreciated by physicians, veterinarians, Crohn’s disease patients, and people who follow this line of research.

Those wanting more background on this subject should visit the page on this site titled Zoonotic Potential where a synopsis of the subject is presented together with a long list of the primary research papers related to the subject and direct links to the full publications.